Yes, but it depends on the computer's operating system (Mac OS or Windows). Sonnet Tempo SATA PCIe, PCI-X, PCI, and ExpressCard/34 cards support drive hot swapping only when used in Mac computers running Mac OS. SATA drive hot swapping is not supported in a desktop or server PC running Windows XP, Server 2003, or Vista. A SATA drive appears to Windows as a fixed hard drive and there is no mechanism in Windows to prepare the drive for removal except in notebook computers.
Tempo SATA and SATA Pro ExpressCard/34 cards support drive hot swapping in both Mac and Windows notebook computers.